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Product Info Summary
| SKU: | LS007041 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of transforming growth factor beta 1 induced transcript 1 (TGFB1I1), transcript variant 2 |
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Product info
Product Name
HIC5 (TGFB1I1) (NM_015927) Human Over-expression Lysate
SKU/Catalog Number
LS007041
Size
100 µg
Description
Transient overexpression lysate of transforming growth factor beta 1 induced transcript 1 (TGFB1I1), transcript variant 2
Storage & Handling
Cite This Product
HIC5 (TGFB1I1) (NM_015927) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS007041)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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HIC5 (TGFB1I1) Human Over-expression Lysates NM_001164719.
Western blot validation of overexpression lysate (LS007041) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TGFB1I1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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HIC5 (TGFB1I1) Human Over-expression Lysates NM_015927.
Western blot validation of overexpression lysate (LS007041) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TGFB1I1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TGFB1I1 (Source: Uniprot.org, NCBI)
Gene Name
TGFB1I1
Full Name
Transforming growth factor beta-1-induced transcript 1 protein
Weight
49.814kDa
Superfamily
paxillin family
Alternative Names
Androgen receptor coactivator 55 kDa protein; androgen receptor coactivator ARA55; Androgen receptor-associated protein of 55 kDa; ARA55; ARA55Hydrogen peroxide-inducible clone 5 protein; HIC5; HIC-5; TGFB1I1; transforming growth factor beta 1 induced transcript 1; transforming growth factor beta-1-induced transcript 1 protein; TSC-5 TGFB1I1 ARA55, HIC-5, HIC5, TSC-5 transforming growth factor beta 1 induced transcript 1 transforming growth factor beta-1-induced transcript 1 protein|androgen receptor coactivator 55 kDa protein|androgen receptor coactivator ARA55|androgen receptor-associated protein of 55 kDa|hydrogen peroxide-inducible clone 5 protein|hydrogen peroxide-inducible clone-5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TGFB1I1, check out the TGFB1I1 Infographic
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In this infographic, you will see the following information for TGFB1I1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For HIC5 (TGFB1I1) (NM_015927) Human Over-expression Lysate (LS007041)
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